• EMBO Practical Course: Techniques for Mammary Gland Research

  • So, 04.03.2018 ab 15:00 Uhr
  • EMBL Events
    Meyerhofstr. 1
    69117 Heidelberg
EMBO Practical Course: Techniques for Mammary Gland Research - EMBL Events - Heidelberg

This theoretical and practical course will present techniques for the dissection and dissociation of different subpopulation of both mouse and human mammary cells, including stem/progenitor cells. It will also include flow cytometry to separate single cell populations for the consequent in vitro (mammosphere and 3D cultures) and in vivo (mouse mammary fat pad clearing and cell transplantation) analysis to assess stem cell activity and gene function. Finally, single cells and cell structures will be analysed by cell imaging, which takes advantage of the extraordinary facilities and expertise at EMBL-HD and constitutes a strong component of the course.